ATGC – Advanced Testing
For Genetic Composition
Single-nucleotide resolution for microbial quantification in research and clinical applications
What is ATGC?
Advanced Testing for Genetic Composition (ATGC) is REM’s proprietary platform for precise microbial quantification. The technology combines Cycling Temperature Capillary Electrophoresis (CTCE) with a curated reference database and specialized bioinformatics algorithms to identify and quantify bacterial and fungal species.
ATGC differentiates closely related organisms that conventional methods cannot reliably distinguish. Whether identifying pathogenic versus commensal species, characterizing specific probiotic strains or detecting antimicrobial resistance variants, ATGC provides quantitative precision down to single-nucleotide differences where genetic variation permits.
Why Single-Nucleotide Resolution Matters?
In microbiome research and clinical microbiology, identification at genus or species-level often masks critical biological differences. Closely related organisms, whether probiotic strains, pathogenic variants or antimicrobial-resistant subtypes, can differ dramatically in function. ATGC quantifies individual organisms with reproducible precision, revealing distinctions that methods measuring total community composition often cannot detect.
How It Works
Platform
Cello 96-capillary electrophoresis system
Detection
Dual-laser, 6-channel fluorescence
Throughput
96 samples per run
Analysis Time
Hours to days (depending on assay and batch size)
Output
Relative abundance measurements for individual species, subspecies and strain variants
Sample Collection
Stool, soil, swabs or other biological matrices collected according to standard procedures
DNA Extraction
Validated extraction protocols optimized for each sample type and assay
Targeted PCR
Amplification of genetic regions containing diagnostic single-nucleotide variants
CTCE Separation
High-resolution detection of single-nucleotide variations via cycling temperature
Quantitative Analysis
Relative quantification of individual species and subspecies
ATGC quantifies defined groups of organisms with high reproducibility across sample sets, supporting both research studies and clinical testing.
Focused Panels
Targets organisms relevant to your specific application, whether distinguishing probiotic strains, identifying pathogens, or tracking specific species in environmental samples.
High Discriminatory Power
Uses SNP-rich regions and CTCE to separate closely related variants that differ by a single nucleotide.
Quantitative Consistency
Each primer undergoes extensive optimization and validation, with strict limits on technical variability.
Scalable Workflow
Uses standard PCR followed by capillary electrophoresis on Cello. Processes up to 96 samples per run using existing laboratory equipment and workflows.
Sequencing-Free
No library preparation or complex bioinformatics required. Results generated directly through REM’s analysis software.
Key Advantages
Quantitative Precision
High reproducibility and low technical variation for relative abundances and strain ratios.
High-Resolution Detection
Single-nucleotide differences distinguish closely related species and subspecies.
Rapid Turnaround
Analysis completes in hours to days, depending on assay and batch size.
No Sequencing Required
Avoids complex library prep and computational bottlenecks
Validated Performance
Assays developed under quality-controlled protocols suitable for clinical and industrial applications.
Continuous Innovation
Each new assay adds to the reference database, improving identification accuracy and reducing custom panel development time.
Precision That Improves With Every Sample
ATGC improves through continuous validation across different sample types. As the reference database expands with each new assay, analysis algorithms become more accurate and custom assays can be developed more quickly for new applications.
Ready to Explore ATGC for Your Application?
Contact us to discuss sample requirements, technical specifications and how ATGC can address your needs.